Natural Bifidobacterium Animalis

PRODUCT DESCRIPTION

Natural Bifidobacterium animalis HH-BAA68 powder contains the Bifidobacterium animalis HH-BAA68 and a special protective agent. This strain was isolated from healthy infant intestine, it can survive in the acidic gastric environment and reach the small intestine and colonize the host. In where, it acts in sevearl ways to enhance health.

SPECIFICAITON

Strain name	Bifidobacterium animalis	Strain number	HH-BAA68
Potency	500bn cfu/g	Gene Identification	Yes
Deposit	CCTCC	Certification	ISO9001/ISO22000/FSSC22000/HACCP/HALAL/KOSHER/FDA
Packing	500g,1kg or 5kg per foil bag	Storage	-18℃ for long time, 2-8℃ for short time

TECHNICAL INDICATORS

Items		Specification
1) Live Bifidobacterium animalis potency		≥ 5.0×1011 CFU/g
2) Water content		≤8.0%
4) Coliforms		≤10CFU/g
5) Moulds, Yeasts		≤10CFU/g
6) Pathogenic bacteria		Negative
7) Heavy metals	Lead (Pb)	≤1ppm
	Arsenic (As)	≤0.5ppm

RELEVANT RESEARCH

Strain Name	Identification	Deposit No.	Antibiotic Susceptibility	Acid and Bile Salt resistance	Stability	Quality System
B.lactis HH-BA68	16S rRNA	-	Done	Done	Done	SC/ISO9001/ISO22000/FSSC22000/HACCP/ HALAL/KOSHER/FDA

ACID TOLERANCE

Acid resistance of Bifidobacterium animalis HH-BAA68 powder was carried out at either pH=2.0 and pH=3.0 for up to 4 hours. The microbial enumeration of Bifidobacterium animalis HH-BAA68 should be performed no more frequently than at the initial(as control), 1 hour, 2 hour, and 4 hour time points. The Fig 1 illustrates that Bifidobacterium animalis HH-BAA68 can survive at low pH conditions, which means this strain has good acid resistance.

Fig 1. Acid resistance of B.lactis HH-BAA68

BILE SALT TOLERANCE

Bile salt resistance of Bifidobacterium animalis HH-BAA68 powder was carried out at either 0.2% and 0.3% bile salt for up to 4 hours. The microbial enumeration of Bifidobacterium animalis HH-BAA68 should be performed no more frequently than at the initial(as control), 1 hour, 2 hour, and 4 hour time points. The Fig 2 illustrates that Bifidobacterium animalis HH-BAA68 can survive at high concentration of bile salt, which means this strain has good bile salt resistance.

Fig 2. Bile salt resistance of B.lactis HH-BAA68

STORAGE STABILITY

Bifidobacterium animalis HH-BAA68 powder were sealed in aluminum foil bag, maintained at -18℃ for 24 months for long-term stability test. The microbial enumeration of Bifidobacterium animalis HH-BAA68 should be performed no more frequently than at the initial, 1, 2, 3, 6, 9, 12, 15, 18, 21, 24 month time points. According to Fig 3, the potency of Bifidobacterium animalis HH-BAA68 powder was 5.41×10¹¹ CFU/g. After a storage period of 24 months, the potency of the powder was 3.98×10¹¹CFU/g. It means that this strain can remain stable for long time if stored at low temperature.

Fig 3. Storage stability of B.lactis HH-BAA68

HEALTH BENEFITS

1. Inhibit pathogen colonization

The aim of this study was evaluation of antagonistic effect of B.lactis HH-BAA68 against colonization by pathogens. The results showed(Table 1) that B.lactis HH-BAA68 is critical in providing resistance against colonization by four pathogenic microorganisms.

	S.aureus	C.difficile	S.typhimurium	L.monocytogenes
Colonization inhibition rate（%）	41.0±2.1	26.4±4.8	4.7±3.1	23.3±5.1
Colonization replacement rate（%）	23.9±5.5	51.3±6.6	6.2±2.1	39.5±6.7

Table 1. Pothogen colonization inhibitory ability of B.lactis HH-BAA68

2. Inhibit pathogen growth

The aim of this study was evaluation of antagonistic effect of B.lactis HH-BAA68 against four pathogens. Antimicrobial effects of isolates were evaluated by disc diffusion test on plates with different pathogens. Growth inhibition zones of pathogens and B.lactis HH-BAA68 inhibitory ability were assessed after incubation of all agar media at 37℃ for 24h. The results showed (Table 2) that B.lactis HH-BAA68 had relatively strong activity against tested pathogens (with inhibition zone diameter> 15mm).

	B.cereus	C.difficile	E.coli	L.monocytogenes
Inhibition zone’s diameter/mm	23.3±1.8	18.7±1.4	23.9±2.1	32.6±3.9

Table 2. B.lactis HH-BAA68 inhibitory ability

APPLICATIONS

Fermented milk; Dietary supplements

Functional foods (Beverages, Bakeries, meal replacement)

PACKING AND DELIVERY

COMPANY IMAGE

http://www.probioway.com/